大花紫薇叶石油醚部位的化学成分研究

作者：詹勤，王燕，李霞，陈万生孙连娜

【摘要】目的研究大花紫薇Lagerstroemia speciosa (Linn.) Pers叶中石油醚部位的化学成分。方法釆用硅胶柱色谱和Sephadex LH-20等分离手段对大花紫薇叶的石油醚部位进行分离纯化，通过ESI-MS、NMR等光谱技术和化学方法鉴定所分离化合物的结构。结果从中分离得到10个化合物，分别为熊果酸(ursolic acid, 1)、2a -经基熊果酸(corosolic acid, 2)、2a -轻基白桦脂

酸（alphitolic acid, 3)、积雪草酸（asiatic acid, 4)、p -谷甾醇（p - sitosterol, 5)、正三十二醇(dotriacontanol, 6)、胡萝卜苷(daucosterol,

主要成分为乙酸芳樟酯、薰衣草醇、芳樟醇、松油醇、香叶醇、橙花醇、樟脑、龙脑、乙酸松油酯、乙酸薰衣草酯、壬醛、蒎烯、月桂烯、罗勒烯等。c黄no.11、二乙醇胺-鲸蜡醇磷酸酯、油酸癸酯、脱氢乙酸、硅藻土、二偶氮利定脲、二异丙基二聚体二亚油酸酯、聚二甲基硅氧烷、聚二甲基硅氧烷共聚醇、聚二甲基硅氧烷醇、透明质酸二甲基硅烷醇酯、己二酸二辛酯、二油基生育酚甲基硅烷醇、一缩二丙二醇、cocoampho二乙酸二钠、乙二胺四乙酸二钠、磷酸二钠、二甲基二羟甲基乙内酰脲、dna、乙二胺四乙酸、弹性蛋白、麦角骨化醇、七叶素、乙氧基二甘醇、亚油酸乙酯、对羟苯甲酸乙酯、蓝桉油、乙酸法呢酯、fd&。水芹烯1.04、对伞花烃0.77、异松油烯1.51、乙酸异辛酯3.32、芳樟醇29.91、乙酸芳樟酯38.04、樟脑0.12、薰衣草醇0.30、乙酸薰衣草酯0.56、&beta。

urs-12-ene-2，3，28-triol，10)]。结论化合物4和10为首次从该植物中分离

得到。

【关键词】大花紫薇；化学成分；结构鉴定

Abstract： Objective To study the chemical constituents of

petroleum ether extract of Lagerstroemia speciosa (Linn.) Pers leaves. MethodsSeparation and purification were performed on silica gel chromatograph and Sephadex LH-20. Their chemical structures were elucidated by chemical methods and spectral data(ESI-MS、

in an other trial, at 16 millimolar scale, similar results were obtained: only pt2 p and pt3 -br could be identified by 1 h-nmr。9. toxicity of four compounds identified as 6a, 12a-dehydro-α-toxicarol, 6a, 12a-dehydro-deguelin, -tephrosin and-6-hydroxy-6a, 12a-dehydro-toxicarol obtained from the leaves and stem of amorpha fruticosa and their mixture in a natural ratio 1:2.54:4.18:2.54 were tested against trematodes grandis.。 methylene chloride as solvent, 4methoxyphenol as inhibitor and n(bps)∶n(mc)=1∶22. the results shows that the reaction conditions were moderate and has high yield(671%). the structure of product was characterized by ir, 1h nmr and gcms.。

acid(l)，corosolic acid(2), alphitolic acid(3)，asiatic acid(4), P -

sitosterol(5)，dotriacontanol(6)，daucosterol(7)，taraxasterol

acetate(8)，(3 -sitosterol acetate(9) and (2a石油醚部位，3P )-urs-12-ene-2，3，28-

triol ， (10) respectively. ConclusionCompounds 4 and 10 were separated from Lagerstroemia speciosa (Linn.) Pers for the first time.

Key words： Lagerstroemia speciosa (Linn.) Pers; Chemical constituents; Structure identification

大花紫薇Lagerstroemia speciosa (Linn.) Pers为千屈菜科紫薇属植物，

又名大叶紫薇、大果紫薇，系一种分布于斯里兰卡、印度、马来西亚、越南及菲律宾等国家的大乔木，在我国广东、广西及福建地区常栽培供观赏[1]。近年来大花紫薇已经引起各国学者的关注石油醚部位，为了充分利用大花紫薇这一潜在药用资源，本文对采集福建漳州地区的大花紫薇叶进行了化学成分研究，现报道其中石油醚部位10个化合物的分离和鉴定结果。

1.1药材样品于2006-09采于福建漳州，经中国人民解放军第二军医大学长征医院药学部陈万生教授鉴定为千屈菜科紫薇属植物大花紫薇Lagerstroemia speciosa (Linn.) Pers 的叶。

1. 2仪器培点用日本Yanaco显微熔点测定仪(温度计未校正）；质谱用Varian MAT-212型质谱仪测定；核磁共振谱用Bruker-speckospin A0600P型

核磁共振仪测定(600 MHz)。

1. 3材料柱层析和薄层层析硅胶均为烟台江友硅胶开发有限公司生