人白血病抑制因子(LIF) (Human)

产品描述:

Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo.

表达系统 :

Escherichia coli

溶解方法:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

产品组成:

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4

存储条件:

This lyophilized preparation is stable at 2-8°C,but should be kept at -20°C for long term storage白血病抑制因子(lif), preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

分子量:

a 547-bp region, containing the n-terminal 153 amino acids of saru and an 88-bp sequence upstream of the translation start, was deleted and replaced with an ~ 1。s production of amino acid food grade, agriculture grade amino acids, amino acid for aquaculture, livestock feed grade amino acids, medicine,...。nitrogen,performed amino acids and protein on microbial activity in the rumen of sheep receiving diets containing rapidly and slowly degraded fiber sources.anim.feed sci.technol. 63:333～340. czerkawski,j. w.1976. chemical composition of microbial matter in the rumen. j.sci.food。

内毒素:

Less than 1 EU/μg of LIF as determined by LAL method.

产品用途:

This material is offered by Cyagen Biosciences for research,laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.

生物学活性:

The specific activity determined by inducing differentiation of murine M1 myeloid leukemic cells. The minimun detectable concentration of LIF in this assay is 0.5 ng/mL白血病抑制因子(lif), corresponding to a specific activity of > 2 × 108 IU/mg, where 50 units is defined as the amount of LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.

产品形态:

Sterile filtered white lyophilized (freeze-dried) powder.

氨基酸序列:

SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF

产品纯度:

> 98% by SDS-PAGE and HPLC analyses.